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. 2018 Jan 26;9:19. doi: 10.3389/fmicb.2018.00019

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Copyright © 2018 Hottmann, Mayer, Tomek, Friedrich, Calvert, Titz, Schäffer and Mayer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Accumulation of MurNAc and UDP-MurNAc-pentapeptide in T. forsythia WT and ΔTf_murK::erm cells in the exponential and stationary phase. Relative amounts of MurNAc and UDP-MurNAc-pentapeptide in extracts of T. forsythia WT (black) and ΔTf_murK::erm cells (gray) were determined by mass spectrometry. Shown are the mean values of three replicates calculated from the area under the curve (AUC) determined for exponential phase cells (adjusted to OD₆₀₀ = 0.5) and stationary phase cells (adjusted to OD₆₀₀ = 1.5).