Table 1.

Oligonucleotide primers used for PCR amplification reactions.

Primer	Sequence (5′-3′)
1068for	GCGCCATGGCGATACTGATTGCAGATAGC
1068rev	GCGCTCGAGTACGGTTTTTGCAACTGTCGAATAG
622	ATAATCCCGGATCATGGTCGTTCG
623	CTTTGCGCACCCGACGAGATGATG
624	TTCAGACGCCGGAAGAGATG
625	GGATTGCGAACGATTGTACC
1068upfor	ACACCGACCGACCTCGTATTTCCTTTC
1068uprev	CGAACGGGCAATTTCTTTTTTGTCATATTTTGATATATATTTTTTTCTTATACAAGAT
1068downfor	GTCCCTGAAAAATTTCATCCTTCGTAGATGACATTTATCAAAATAACAGAACAGG
1068downrev	GGTAAGCGGTCATCATCTCTCGTCGG
524	GTAAAACGAACGGGCAATTTCTTTTTTGTCAT
525	CCCTGAAAAATTTCATCCTTCGTAG
460	ATGACAAAAAAGAAATTGCCCGTTCGTTTTAC
461	CTACGAAGGATGAAATTTTTCAGGGACAAC

Nucleotides used for overlap-extension PCRs are written in bold, artificially introduced restriction enzyme sites are italicized.