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Kinetic parameters of Tf_MurK.
| Substrate | KM [μM] | Vmax [μmol min-1 mg-1] | kcat [s-1] | kcat/KM [s-1 M-1] | Ki(S) [mM] |
|---|---|---|---|---|---|
| Kinetic parameters fitted to Michaelis-Menten equation: | |||||
| MurNAc | 113 | 39.5 | 7.9 | 69910 | nd |
| GlcNAc | 116700 | 0.5 | 0.1 | 0.86 | nd |
| Kinetic parameters fitted considering substrate inhibition: | |||||
| MurNAc | 200 | 52.6 | 10.5 | 52550 | 4.2 |
nd, not determined.
