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. 2018 Jan 26;9:53. doi: 10.3389/fpls.2018.00053

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Copyright © 2018 Ren, Zhang, Wang, Liu, Li, Wang and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Construction of the pBtiA plant expression vector containing API-A and Cry1Ac genes. Nos pro, promoter of neomycin phosphotransferase gene; nptII, neomycin phosphotransferase gene; Nos ter, terminator of neomycin phosphotransferase gene; 35S, cauliflower mosaic virus 35S promoter; API-A, arrowhead proteinase inhibitor gene; Cry1Ac, Bacillus thuringiensis insecticidal crystal protein gene (Tian et al., 2000).