Table 7.
Aspergillus isolates identity using three genes and aflatoxin production.
| Culture media | Aflatoxin produced | Isolate identity from NBCI blast | Grouping of isolate identity from NCBI database using various genes | N total | ||
|---|---|---|---|---|---|---|
| β-tubulin | Calmodulin | ITS | ||||
| CYA | Both AFB's and AFG's | A minisclerotigenes | 4, 10, 54, 67, | 27 | ||
| A. flavus | 2, 5, 8, 11, 12, 13, 14, 16, 17, 18, 26, 29, 36, 40, 46, 47, 58, 68, 72, 77, 93, 102, 105 | 2, 4, 5, 8, 10, 11, 12, 13, 14, 16, 17, 18, 26, 29, 36, 40, 46, 47, 54, 58, 67, 68, 72, 77, 93, 102, 105 | 2, 4, 5, 8, 10, 11, 12, 13, 14, 16, 17, 18, 26, 29, 36, 40, 46, 47, 54, 58, 67, 68, 72, 77, 93, 102, 105 | |||
| AFB's | A minisclerotigenes | 1, 30, 37, 42, 84 | 1, 30, 37 | 66 | ||
| A. flavus | 3, 6, 7, 9, 15, 19, 20, 21, 25, 27, 28, 33, 34, 35, 38, 39, 41, 42, 44, 45, 49, 50, 52, 53, 55, 57, 59, 61, 64, 65, 66, 69, 70, 71, 73, 74, 75, 78, 80, 81, 82, 83, 84, 85, 86, 87, 89, 91, 92, 94, 95, 96, 97, 98, 99, 100, 101, 103, 104, 106, 107, 108 | 1, 3, 6, 7, 9, 15, 19, 20, 21, 25, 27, 28, 30, 33, 34, 35, 37, 38, 39, 41, 42, 44, 45, 49, 50, 52, 53, 55, 57, 59, 61, 64, 65, 66, 69, 70, 71, 73, 74, 75, 78, 80, 81, 82, 83, 84, 85, 86, 87, 89, 90, 91, 92, 94, 95, 96, 97, 98, 99, 100, 101, 103, 104, 106, 107, 108 | ||||
| A. parasiticus | 90 | 90 | ||||
| Unnamed. Only 99% match of A. flavus in NCBI database | 1, 28 | |||||
| No aflatoxin | A. flavus | 63 | 63 | 63 | 1 | |
| YESA | Both AFB's and AFG's | A minisclerotigenes | 1 | 1 | 6 | |
| A. flavus | 11, 82, 93, 102 | 11, 82, 93, 102 | 11, 82, 93, 102 | |||
| A. parasiticus | 90 | 90, | 90 | |||
| Unnamed. Only 99% match of A. flavus in NCBI database | 1 | |||||
| AFB's | A minisclerotigenes | 4, 10, 30, 37, 42, 54, 67, 84 | 30, 37 | 85 | ||
| A. flavus | 2, 3, 5, 6, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 26, 27, 28, 29, 33, 34, 35, 36, 38, 39, 40, 44, 45, 47, 48, 49, 50, 52, 53, 55, 57, 58, 59, 61, 63, 64, 65, 66, 68, 69, 70, 71, 72, 73, 75, 76, 77, 78, 80, 81, 83, 85, 86, 87, 89, 91, 92, 94, 95, 96, 97, 98, 99, 100, 101, 103, 104, 105, 106, 107 | 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 26, 27, 28, 29, 33, 34, 35, 36, 38, 39, 40, 42, 44, 45, 47, 48, 49, 50, 52, 53, 54, 55, 57, 58, 59, 61, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 75, 76, 77, 78, 80, 81, 83, 84, 85, 86, 87, 89, 91, 92, 94, 95, 96, 97, 98, 99, 100, 101, 103, 104, 105, 106, 107 | 2, 3, 4, 5, 6, 7, 8, 9, 10, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 26, 27, 29, 30, 33, 34, 35, 36, 37, 38, 39, 40, 42, 44, 45, 47, 48, 49, 50, 52, 53, 54, 55, 57, 58, 59, 61, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 75, 76, 77, 78, 80, 81, 83, 84, 85, 86, 87 89, 91, 92, 94, 95, 96, 97, 98, 99, 100, 101, 103, 104, 105, 106, 107 | |||
| Unnamed. Only 99% match of A. flavus in NCBI database | 28 | |||||
| No aflatoxins | A. flavus | 21, 41, 74, 108, 109 | 21, 41, 74, 108, 109 | 21, 41, 74, 108, 109 | 5 | |