FIG 5 .

Effects of QStatin on SmcR regulon expression. (a) The fold changes in expression of each gene in either the QStatin-treated WT or DMSO-treated ΔsmcR mutant strain are presented relative to those in the DMSO-treated WT strain. Error bars represent the SD of results from three independent experiments. (b) The fold changes of whole transcriptome expression in the DMSO-treated ΔsmcR mutant, QStatin-treated ΔsmcR mutant, and QStatin-treated WT biofilms relative to that in the DMSO-treated WT biofilm were examined by RNA sequencing. Among the genes differentially expressed in DMSO-treated ΔsmcR mutant biofilm relative to DMSO-treated WT biofilm (P ≤ 0.05; fold change, ≥2), 19 genes potentially involved in virulence, motility, and biofilm formation/dispersion were selected. Fold changes of the expression of 19 genes in the indicated samples relative to those in the DMSO-treated WT biofilm are shown in the heat map with colors representing the log₂ RPKM ratio. Locus tags of genes in the V. vulnificus MO6-24/O genome (GenBank accession numbers CP002469.1 and CP002470.1) and their gene products are shown. Please refer to Fig. S4 and Data Set S1 for expression changes in other genes. (c) Principal-component analysis of the whole-gene expression profiles of the samples. Each symbol represents the transcriptome of a single sample from two biological replicates per sample group.