TABLE 1 .
Lowest common ancestor classification and characteristics of predicted steroid degradation MAGsa
| Global environment |
MAG ID | Lowest common ancestor |
Comp- lete- ness (%) |
Conta- mina- tion (%) |
Total length (bp) |
No. of contigs |
N50 | No. of proteins |
Pathway comp- lete- nessb |
Steroid degrader MAGc |
|---|---|---|---|---|---|---|---|---|---|---|
| Aerobic WWTPs | WWT_1.17 | f__Sphingomonadaceae | 53.79 | 0 | 3,185,176 | 607 | 5,709 | 3,363 | 7 | Yes |
| WWT_2.6 | f__Oceanospirillaceae | 68.49 | 2.94 | 2,676,165 | 573 | 4,917 | 3,072 | 8 | Yes | |
| WWT_4.10 | f__Oceanospirillaceae | 75.87 | 5.85 | 4,006,600 | 673 | 6,893 | 4,337 | 9 | Yes | |
| WWT_7.32 | p__Actinobacteria | 96.58 | 8.12 | 5,572,474 | 249 | 85,999 | 5,464 | 9 | No | |
| WWT_7.3 | p__Actinobacteria | 93.16 | 9.83 | 4,233,906 | 207 | 626,926 | 4,650 | 6 | No | |
| WWT_7.2 | c__Betaproteobacteria | 62.73 | 6.23 | 3,448,847 | 701 | 5,345 | 3,900 | 6 | Yes | |
| WWT_13.7 | f__Rhodocyclaceae | 51.23 | 9.13 | 3,553,647 | 538 | 7,567 | 3,957 | 6 | Yes | |
| WWT_19.1 | g__Pseudomonas | 53.45 | 0 | 4,731,881 | 602 | 9,957 | 4,775 | 8 | No | |
| WWT_18.119 | g__Nocardioides | 53.23 | 9.2 | 2,969,492 | 395 | 9,565 | 3,110 | 8 | Yes | |
| Soil | ||||||||||
| Peat | SOI_6.11 | c__Actinobacteria | 33.62 | 7.76 | 4,547,698 | 979 | 4,753 | 5,194 | 6 | No |
| SOI_6.79 | c__Alphaproteobacteria | 47.3 | 5.17 | 2,223,633 | 349 | 7,406 | 2,383 | 6 | Yes | |
| Temperate forest | SOI_9.15 | g__Mycobacterium | 26.84 | 3.23 | 1,057,590 | 253 | 4,160 | 1,168 | 7 | Yes |
| Antarctic Dry Valley | SOI_11.1 | g__Rhodococcus | 99.94 | 5.03 | 7,116,951 | 114 | 122,177 | 6,768 | 10 | Yes |
| SOI_12.1 | g__Rhodococcus | 99.94 | 1.13 | 6,989,039 | 107 | 137,512 | 6,664 | 10 | Yes | |
| Deep ocean | DEO_2.4 | g__Rhodococcus | 85.92 | 2.79 | 5,065,927 | 753 | 8,048 | 5,413 | 10 | Yes |
| DEO_3.1 | g__Rhodococcus | 66.99 | 1.17 | 3,883,956 | 805 | 4,953 | 4,325 | 9 | Yes | |
| DEO_4.4 | g__Rhodococcus | 91.76 | 2.71 | 4,585,835 | 544 | 10,691 | 4,668 | 10 | Yes | |
| DEO_6.5 | g__Rhodococcus | 61.53 | 1.84 | 3,607,454 | 817 | 4,526 | 4,137 | 9 | Yes | |
| DEO_7.4 | g__Rhodococcus | 82.37 | 1.89 | 3,841,856 | 289 | 20,213 | 3,817 | 10 | Yes | |
| DEO_7.5 | f__Nocardioidaceae | 77.5 | 1.99 | 2,700,782 | 549 | 5,207 | 2,967 | 8 | Yes | |
| DEO_8.1 | g__Rhodococcus | 98.98 | 2.71 | 5,531,222 | 381 | 21,692 | 5,499 | 10 | Yes | |
| DEO_9.5 | g__Rhodococcus | 82.35 | 1.02 | 3,377,590 | 267 | 16,836 | 3,281 | 8 | Yes | |
| OPO_7.56 | f__Rhodobacteraceae | 41.06 | 5.73 | 2,641,776 | 244 | 32,960 | 2,696 | 7 | Yes | |
| OMZ | OMZ_4.28 | p__Proteobacteria | 81.01 | 9.87 | 3,893,890 | 574 | 8,445 | 4,045 | 9 | Yes |
| OMZ_5.19 | p__Proteobacteria | 39.71 | 1.41 | 1,628,638 | 363 | 4,714 | 1,815 | 6 | Yes | |
| Lentic | LEN_1.3 | d__Bacteria | 64.23 | 2.47 | 2,391,978 | 554 | 4,404 | 2,323 | 9 | Yes |
| Thermal spring | THS_1.35 | g__Mycobacterium | 41.78 | 3.64 | 3,007,048 | 652 | 4,595 | 3,358 | 6 | Yes |
| THS_1.174 | g__Mycobacterium | 88.91 | 8.27 | 4,404,794 | 590 | 10,309 | 4,448 | 10 | Yes | |
| Deep subsurface | DES_1.10 | f__Rhodobacteraceae | 82.53 | 3.61 | 2,393,238 | 346 | 8,887 | 2,542 | 7 | Yes |
| Sponges | ||||||||||
| Sarcotragus | MSP_1.34 | p__Actinobacteria | 94.02 | 2.56 | 3,648,331 | 87 | 110,111 | 3,524 | 7 | Yes |
| MSP_1.33 | p__Proteobacteria | 95.27 | 3.73 | 4,164,014 | 217 | 45,254 | 3,959 | 7 | Yes | |
| MSP_1.27 | p__Proteobacteria | 84.12 | 1.92 | 2,651,844 | 353 | 8,944 | 2,614 | 7 | Yes | |
| MSP_1.38 | p__Proteobacteria | 99.35 | 4.6 | 4,649,636 | 270 | 40,894 | 4,439 | 6 | Yes | |
| MSP_1.29 | p__Proteobacteria | 97.51 | 1.74 | 3,815,537 | 194 | 30,307 | 3,691 | 6 | Yes | |
| Petrosia | MSP_2.29 | d__Bacteria | 60.26 | 2.56 | 2,480,936 | 132 | 33,781 | 2,374 | 7 | Yes |
| MSP_2.40 | p__Proteobacteria | 57.15 | 1.71 | 2,281,694 | 468 | 5,157 | 2,455 | 7 | Yes | |
| Aplysina | MSP_4.19 | d__Bacteria | 94.44 | 0.85 | 4,165,649 | 63 | 132,205 | 3,873 | 6 | Yes |
| MSP_4.73 | d__Bacteria | 93.03 | 3.53 | 5,571,763 | 126 | 96,451 | 5,617 | 8 | Yes | |
| MSP_4.39 | p__Actinobacteria | 92.59 | 3.42 | 3,973,924 | 264 | 31,801 | 3,913 | 6 | Yes | |
| MSP_4.14 | p__Proteobacteria | 92.62 | 0.5 | 4,493,106 | 192 | 44,772 | 4,413 | 9 | Yes | |
| MSP_4.50 | p__Proteobacteria | 91.28 | 5.43 | 4,468,188 | 348 | 21,113 | 4,308 | 9 | Yes | |
| Rhizosphere | RHI_3.1 | o__Sphingomonadales | 31.3 | 5.17 | 3,865,039 | 737 | 5,362 | 4,414 | 8 | Yes |
| RHI_3.9 | f__Sphingomonadaceae | 67.79 | 1.41 | 2,455,695 | 464 | 5,978 | 2,678 | 8 | Yes | |
| RHI_4.8 | g__Mycobacterium | 32.26 | 0.97 | 2,044,882 | 586 | 3,318 | 2,352 | 7 | Yes | |
| RHI_5.1 | f__Sphingomonadaceae | 83.88 | 7.59 | 3,936,626 | 718 | 6,077 | 4,370 | 8 | Yes | |
| RHI_6.17 | o__Sphingomonadales | 32.92 | 4.31 | 3,995,136 | 807 | 5,052 | 4,300 | 7 | Yes | |
| RHI_6.7 | f__Sphingomonadaceae | 81.88 | 2.18 | 3,031,208 | 564 | 5,796 | 3,353 | 7 | Yes | |
| RHI_7.12 | o__Sphingomonadales | 35.68 | 7.82 | 2,555,306 | 395 | 17,189 | 2,663 | 9 | Yes | |
| Termite gut | TER_1.13 | c__Alphaproteobacteria | 57.76 | 9.25 | 4,007,481 | 892 | 4,701 | 4,256 | 8 | Yes |
a
Listed are 49 metagenome-assembled genomes (MAGs) of predicted steroid degraders.
b
Out of 10 protein families.
c
Based on the presence of orthologs to characterized steroid degradation proteins identified by reciprocal BLASTp.