FIG 3.

NAP subcellular localization and anti-HDV activity in Huh-106 cells. Cells were inoculated with HDV in the presence of Cy3-tagged versions of REP 2031, REP 2055, REP 2139, REP 2147, and REP 2172 at 0.25 μM. Infected cells were counted at 9 dpi using a human anti-HDAg antibody-positive serum and Alexa Fluor 488-conjugated goat anti-human IgGs (green) (B). NAPs were detected at 9 dpi by virtue of their fluorescent tag (red), and nuclei were labeled with DAPI (blue) (A). Note that because spectral overlaps between Cy3 fluorophore (red) and Alex Fluor 488 (green), the yellow spots present in the merge panel demonstrate not colocalization between NAPs and HDAg but Cy3 emission detected in the Alexa Fluor 488 channel. Scale bars, 10 μm. The ratio of infected cells was plotted as percent HDV-infected cells (C). Asterisk, P < 0.05.