FIG 4.
N9 MAbs inhibit H6N9AH/13 plaque formation. MAbs 1E8, 2F6, 10F4, and 11B2 did not affect plaque formation when supplemented in the virus inoculum but inhibited plaque formation when supplemented in agar overlay after cells were infected with virus. (A) MDCK cells growing in six-well plates were infected with ∼30 to 40 PFU of H6N9AH/13 premixed with each MAb at 5 μg/ml and covered with agar without antibody after thorough washing (upper panel) or infected with H6N9AH/13 and then covered with agar containing antibody (5 μg/ml) after washing (lower panel). Control, N1-specific MAb 3A2. (B) MDCK cells were infected with H6N9AH/13 and then overlaid with agar that contained various concentrations of MAbs 1E8, 2F6, 10F4, and 11B2 (0.1 to 10 μg/ml) or the control MAb, 3A2 (10 μg/ml).