FIG 2.

Components of exosomes derived from PRRSV-infected cells. (A) Purified exosomes derived from PRRSV-infected cells were analyzed with LC-MS/MS to determine the host proteins and viral proteins present. Classification of host proteins according to function is shown. (B) PRRSV proteins in exosomes were confirmed on Western blots probed with antibody directed against M, N, GP5, GP4, or nsp2. (C) PRRSV genomic RNAs in exosomes isolated from PRRSV-infected cells were detected with RT-PCR. Five overlapping fragments (A, B, C, D, and E) were designed based on the genome sequence of PRRSV strain WUH3.