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Time course analysis of Ac75 expression. Sf9 cells were mock infected or infected with vAc75:FLAG at an MOI of 10 TCID50/cell. At the indicated time points, the cells were collected, resolved by SDS-12% PAGE, and analyzed by immunoblotting with a monoclonal anti-FLAG, -GP64, or -actin antibody to detect Ac75, GP64, or actin. Mi, mock-infected cells. The molecular masses (in kDa) of protein standards are indicated on the left.
