FIG 8.

Distribution of Ac75 following phase separation. Sf9 cells were infected with vAc75:FLAG at an MOI of >10 TCID₅₀/cell. At 72 h p.i., the cells were collected, extracted with 1% Triton X-114, and analyzed by Western blotting with a mouse monoclonal anti-FLAG or anti-GP64 antibody as the primary antibody and a goat anti-mouse HRP-conjugated antibody as the secondary antibody to detect Ac75 or GP64. Lanes: C, cell lysates; D, detergent phase; A, aqueous phase. The molecular masses (in kDa) of protein standards are noted on the left.