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. 2018 Jan 30;92(4):e02019-17. doi: 10.1128/JVI.02019-17

FIG 1.

FIG 1

HUVEC are permissive for infection with ZIKV strains of both the African and Asian/American lineages. (A to D) Growth kinetics of ZIKV strains IbH 30656 (A), MR766 (B), H/FP/2013 (C), and PRVABC59 (D) in HUVEC. HUVEC were infected with ZIKV at an MOI of 1.0 for 1 h and then washed three times with PBS. The culture medium was replaced, and the supernatant was collected at 3, 24, 48, 72, and 96 h for plaque assay. (E to H) Kinetics of genome replication of ZIKV strains IbH 30656 (E), MR766 (F), H/FP/2013 (G), and PRVABC59 (H). ZIKV-infected HUVEC were lysed at the indicated time points. Both positive- and negative-sense viral genomes were measured by RT-qPCR. (I to L) Kinetics of viral protein synthesis of ZIKV strains IbH 30656 (I), MR766 (J), H/FP/2013 (K), and PRVABC59 (L). ZIKV-infected HUVEC were lysed at the indicated time points. Both nonstructural (NS1) and structural (capsid) viral proteins were measured by Western blotting.