FIG 2.

Histo-blood group A and B phenotype dependence of the binding of lagovirus strains to duodenum extracts of rabbits (O. cuniculus). VLPs from each strain were incubated on ∼3-μg/ml protein-coated tissue extracts from animals of the A⁻ B⁻, A⁺ B⁻, and A⁺ B⁺ phenotypes, and their binding was quantified by ELISA. To account for variations in extract material concentrations, the data points correspond to normalized mean OD values for each animal. For GI.1d (RHDV G5), the values were normalized to ConA binding values as previously described (59). For the other strains, OD values were normalized to the protein concentrations of the extracts. The horizontal bars represent mean values and standard deviations (SD). Statistically significant differences between groups are indicated: **, P < 0.01; ***, P < 0.0001 (two-tailed Mann-Whitney test).