Figure 2.

Common brush border antigen among ABBA sera but not negative controls. (A) ABBA⁺ serum stains renal tubular brush border (indirect immunofluorescence using normal human kidney tissue, ×100). (B) Western blot (WB) of HTE with individual patient sera (lanes 1–4) or control sera (lanes 5–8); secondary antibody is sheep anti-human IgG1 for lane 1 and sheep anti-human IgG4 for lanes 2–8. (C) HTE was electrophoresed under nonreducing (NR) versus reducing conditions (red); then; it was Western blotted with ABBA⁺ serum (1:100) and detected with sheep anti-human IgG4.