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. Author manuscript; available in PMC: 2018 Jan 31.
Published in final edited form as: Nat Immunol. 2016 Aug 22;17(10):1159–1166. doi: 10.1038/ni.3523

Figure 6.

Figure 6

CD1a binds and displays urushiol (C15:2) in its antigen-binding cleft. (a) Molecular interactions of urushiol (C15:2) with CD1a. Superposition of the CD1a-urushiol binary complex (in grey) and the complex of TCR-CD1a-endogenous lipid (in light blue) (PDB code: 5J1A, 4X6D). (b) Extracted ion chromatograms (Q1-Q3 transition) of urushiol C15:2 synthetic standard (in black), purified recombinant CD1a loaded with urushiol (in green), and crystal of CD1a-urushiol C15:2 (in red). (c) Accurate mass spectra for urushiol extracts comparing predicted molecular weight and isotope distribution pattern. urushiol C15:2 theoretical mass (c, top), urushiol C15:2 synthetic standard (c, upper middle), purified CD1a-urushiol C15:2 soluble extract (c, lower middle), and CD1a-urushiol C15:2 binary complex crystal (c, bottom).