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. 2018 Jan 24;24:461–472. doi: 10.12659/MSM.908430

Figure 3.

Figure 3

RNAi of DNMT1/3b and HDAC1/3 inhibit CSLCs self-renewal. (A) Western blot analysis of DNMT1 and DNMT3b protein expression with the shRNA interference (shDNMT1, shDNMT3b vs. shNC). (B) Western blot analysis of HDAC1 and HDAC3 protein expression with the shRNA interference (shHDAC1, shHDAC3 vs. shNC). (C) Sphere counts in first- and second-generation in cells with overexpressing transfection of DNMT1, DNMT3, HDAC1, and HDAC3 (co-expression for 4 lentivirus transfections together; groups of shRNA vs. shNC group). (D) After the transfection, stemness-associate genes in the sphere cells were detected by RT-PCR (groups of shRNA vs. shNC group). Data represent mean ±SD from at least 3 independent experiments, * p<0.05; ** p<0.01; *** p<0.001.