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. 2018 Jan 24;24:461–472. doi: 10.12659/MSM.908430

Figure 6.

Figure 6

Curcumol affects CSLCs via regulation of DNMT and HDAC. With the treatment of curcumol combined with 5-AzaC or TSA, (A) the numbers of spheres in first- and second-generation cultures from JEG-3 cells (all groups vs. DMSO group; 0 μM curcumol+5-AzaC+TSA vs. 75 μg/ml curcumol+DMSO) and (B) stemness-associated genes mRNA expression levels. With the transfection of over-expression lentivirus of DNMT1 or HDAC1, (C) Western blot analysis of DNMT1 and HDAC1 protein levels (left) and respective changes (right), (D) sphere counts in first- and second-generation in cells (over-expression groups vs. Vector group) and (E) respective stemness-associated genes mRNA expression levels. Data represent mean ±SD from at least 3 independent experiments, * p<0.05; ** p<0.01; *** p<0.001.