Figure 2. pSOL™ Expression Vectors.

A suite of eight vectors, each containing a unique fusion partner, enables instant parallel cloning of a single amplicon containing the target of interest. The target gene amplicon is flanked by unique 18 bp sequences at each end that are homologous to the universal ends of the eight vectors. In the Expresso cloning method, the amplicon is simply combined with one of the eight vectors and transformed immediately into chemically competent E. coli. Endogenous recombination activity within the cells fuses the insert to the vector seamlessly. The amino-terminal cassette in each vector contains a translational start codon, 6×His and fusion tag sequence joined to the target sequence by a TEV Protease cleavage site, enabling IMAC purification and TEV Protease-mediated tag removal. A rhamnose-inducible promoter provides high-level, inducible protein expression of the target fusion. A strong transcriptional terminator prevents unwanted transcription into or out of the cloned sequence.