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A gene encoding the GH1 (human growth hormone) isoform from pituitary was cloned into the 8 pSOL vectors and evaluated for expression and solubility. Improved soluble expression was observed for each of the seven fusion tags compared to the 6×His control. Several fusion partners, especially Tsf, Bla, and MBP also promoted a dramatic increase in protein expression compared to the control. T: Total Lysate, S: Cleared Soluble Fraction, I: Insoluble Pellet.
