Skip to main content
. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Curr Protoc Protein Sci. 2017 Nov 1;90:5.27.1–5.27.20. doi: 10.1002/cpps.39

Figure 4. Fusion-tag removal and purification of detagged GH1.

Figure 4

A. Lanes 1 and 2 show purified Bla-GH1 fusion protein before and after cleavage with SelecTEV protease. Lanes 3, 4 and 5 show flow-through, wash and elution fractions from IMAC purification following the cleavage reaction. Free GH1 was present in the flow-through. The 6×His-tagged Bla fragment and SelecTEV protease are retained on the column and released by elution with buffer containing 0.25 M imidazole. M, marker proteins.

B. Purified, detagged GH1 remained soluble upon centrifugation after 72 h at 4 °C.