. Author manuscript; available in PMC: 2018 Nov 1.

Published in final edited form as: Curr Protoc Protein Sci. 2017 Nov 1;90:5.27.1–5.27.20. doi: 10.1002/cpps.39

Table 1.

Expected Sizes of Colony PCR Amplicons.

Vector	pRham Forward + pETite Reverse^*	pRham Forward + GOI Reverse^*
pSol AFV	490	439
pSol SlyD	781	730
pSol Tsf	1042	991
pSol SUMO	496	445
pSol Bla	1294	1243
pSol MBP	1297	1246
pSol GST	850	799
pSol His Control	193	142

Size, in base-pairs, of the vector portion of an amplicon produced with the specified primers. To determine the expected size of the entire amplicon, the length of the gene of interest must be added to the value in the table.