Table 2.
Amino Acid Length and Molecular Weight of Fusion Tags in pSOL vectors
| Fusion Tag | AA Length | kDa* | pI | Description |
|---|---|---|---|---|
| 6×His-AFV | 113 | 13.5 | 5.0 | AFV1–99 protein from Acidianus filamentous virus 1 |
| 6×His-SlyD | 210 | 22.7 | 5.2 | FKBP-type peptidyl-prolyl cis-trans isomerase |
| 6×His-Tsf | 297 | 32.2 | 5.7 | E. coli elongation factor |
| 6×His-SUMO | 115 | 13.3 | 5.2 | Small Ubiquitin-like Modifier |
| 6×His-Bla | 381 | 41.3 | 4.4 | Beta-lactamase |
| 6×His-MBP | 382 | 42.1 | 5.5 | Maltose-Binding Protein |
| 6×His-GST | 233 | 27.4 | 6.6 | Glutathione S-transferase |
| 6×His Control | 14 | 1.8 | 7.0 | Affinity tag |
*
Molecular weights listed are all based on amino acid composition. Some tags (e.g. SUMO and Bla) frequently display anomalous migrations on SDS-PAGE.