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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Curr Protoc Protein Sci. 2017 Nov 1;90:5.27.1–5.27.20. doi: 10.1002/cpps.39
Volume, µL Component Final Concentration/Amount
X Fusion Protein 30 µg
5 SelecTEV 20× Buffer 1×
1 DTT, 100mM 1 mM
1 SelecTEV Protease, 10 U/ µL 10 U
Y Water N/A
100 µL Total