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. Author manuscript; available in PMC: 2018 Jan 31.
Published in final edited form as: Chem Rev. 2015 Aug 10;115(19):10690–10724. doi: 10.1021/cr500698d

Figure 19. Magnetic barcode assay for bacterial detection.

Figure 19

(a) Assay procedure. From unprocessed specimen, bacterial 16sRNA or DNA is extracted though an off-chip stressing. Extracted DNA samples are then loaded into a fluidic device. The target DNA sequences are amplified by asymmetric RT-PCR and captured by polymer beads modified with capture DNA. MNPs are then used to specifically coat the beads via complementary sequences, and the samples are subjected to NMR measurements. (b) A fluidic cartridge was developed to streamline the assay. The device integrates PCR chambers, mixing channels and a microcoil for NMR measurements. (c) Mycobacterium tuberculosis targets in sputum samples were detected. The sensitivity was 102 – 103 CFU/mL. CFU, colony forming unit. Reproduced with permission from Ref. 52. Copyright 2013 Nature Publishing Group.