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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Cancer Res. 2017 Sep 14;15(12):1741–1751. doi: 10.1158/1541-7786.MCR-17-0010

Figure 4. PRELID1 knockdown increases mitochondrial ROS production in ER− breast cancer cells.

Figure 4

A. MitoSOX Red fluorescence (x-axis) vs. proportion of gated events (y-axis) for MCF-7 shRNA control (dark grey) vs. MDA-MB-231 shRNA control (light grey; representative clones). B. MitoSOX Red fluorescence (x-axis) vs. proportion of gated events (y-axis) for MCF-7 shRNA control (dark grey) vs. MCF-7 PRELID1 shRNA knockdown (light grey; representative clones). C. MitoSOX Red fluorescence (x-axis) vs. proportion of gated events (y-axis) for MDA-MB-231 shRNA control (dark grey) vs. MDA-MB-231 PRELID1 shRNA knockdown (light grey; representative clones). D. Relative MitoSOX fluorescence (per-clone median) by shRNA group. Flow was performed on three separate clonal cell lines per group on two separate occasions. Histograms are from representative runs. A Two-way ANOVA (cell line x shRNA) followed by Tukey’s post hoc test was used to determine significance. *p<0.05, **p<0.01, ***p<0.001