Figure 5.

Treatment with CRAMP reduces protein levels of pro-inflammatory cytokines in colon tissues. (A) TNF-α, (B) IL-4, and (C) IL-13 were reduced and (D) IFN-γ level was increased by CRAMP administration at a dose of 20 mg/kg bodyweight. (E) The level of IL-33 was elevated by oxazolone challenge but wasn't affected by CRAMP administration. Cytokines levels in the homogenates of colon tissues were measured by ELISA and standardized with total protein concentrations. Statistical significance was analyzed by one-way ANOVA followed by Newman-keuls multiple comparisons test, ***p < 0.001, **p < 0.01, *p < 0.05, n = 5 – 6; ^#p < 0.05, comparing 20 mg/kg (n = 6) with 5 mg/kg CRAMP (n = 5) by unpaired Student's t-test.