Figure 4.

Fgf Signaling Represses Endoderm Specification around Ndr1 Clones

(A) Animal views of DMSO- and PD-0325901-treated germ ring-stage embryos containing Ndr1-expressing clones stained for lft2 and sox32.

(B) Quantification of sox32-positive cells in (A). Means ± SD, Mann-Whitney U test; ^∗p < 0.05.

(C) Quantification of surface area of lft2-positive domain surrounding Ndr1-expressing clones in embryos treated as in (A). n = 3, 11 embryos in total. Means ± SD, two-tailed t test; ^∗p < 0.05.

(D) Animal views of germ ring-stage embryos treated as in (A), but stained for ta and lft2.

(E) Animal views of germ ring-stage embryos treated as in (A), but stained for noto and lft2.

Scale bars, 100 μm. See also Figure S4.