Figure 4.

Plasticity of Functional Relations in Modules after Pathway Activation

(A) State-specific cumulative rank-based distance to the six destruction complex components Axn, CkIα, slmb, sgg, skpA, and lin19.

(B) Between-state cumulative rank-based distance from the destruction complex. The rank of the PCC of each gene with a destruction complex component was computed and ranks were summed per gene.

(C) Comparison of baseline-specific similarity (PCC between genetic interaction profiles) with baseline and Wnt-active state similarity of baseline state Axn with other destruction complex components (red) or negative feedback regulators (nkd, Notum) (orange).

(D) Between-state relations among components of stable functional modules. Stable functional relations exceeded a connection specificity index (CSI) of 0.9 in the baseline and respective activated state. Self-correlation of all tested genes is illustrated in black; the dark-blue line represents the self-correlation of genes in stable modules.

(E) Between-state relations among components state-specific functional modules. State-specific relations exceeded a CSI of 0.9 in either the baseline or activated state but not in the respective other state (CSI < 0.7). Self-correlation of all tested genes is illustrated in black. Curves were compared using a one-sided Wilcoxon rank-sum test.