REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-Wg	Developmental System Hybridoma Bank	N/A
Guinea Pig anti-Sens	Gross et al., 2012	N/A
anti-guinea pig-Alexa594	Invitrogen	Cat #: A11076; RRID: AB_2534073
anti-mouse-Alexa594	Invitrogen	Cat #: A11005; RRID: AB_2534120
Recombinant DNA
Plasmid: pAc-wg	Bartscherer et al., 2006	N/A
Plasmid: dTCF-luc	Bartscherer et al., 2006	N/A
Plasmid: Rp128-Rluc	Bartscherer et al., 2006	N/A
Deposited Data
Primer for dsRNAs targeting all query and template genes	This paper	Tables S1 and S2
Documented R code vignette	This paper	Data S1
Raw data	https://github.com/boutroslab/Supplemental-Material	WntSGI
Processed data	https://github.com/boutroslab/Supplemental-Material	WntSGI
Experimental Models: Cell Lines
D. melanogaster: Cell line S2R+	Drosophila Genomics Resource Center	FBtc0000150
D. melanogaster: 1182-4H	Drosophila Genomics Resource Center	FBtc0000177
Experimental Models: Organisms/Strains
Drosophila: en-GAL4, UAS-GFP 2nd chr.	Thompson and Cohen, 2006	N/A
Drosophila: Catsup mutant alleles Catsup47	Groth et al., 2013	N/A
Drosophila: RNAi line targeting Catsup	Vienna Drosophila RNAi Center	transformant ID: 103630
Drosophila: fz3-GFP	Bloomington Drosophila Stock Center	Bloomington stock number 43831
Software and Algorithms
R	www.r-project.org	N/A
Custom R code	https://github.com/boutroslab/Supplemental-Material	WntSGI