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. Author manuscript; available in PMC: 2018 Jan 31.
Published in final edited form as: Immunol Cell Biol. 2016 Aug 16;95(1):108–116. doi: 10.1038/icb.2016.73

FIGURE 4.

FIGURE 4

MyD88 and Src interaction in HAdV-D37 infection. Western blot analysis of WT and MyD88−/− mouse corneal lysates at 1 hpi (A) show increased phospho-Src in WT corneas infected with HAdV-D37, but not in MyD88−/− corneas. Infected HCF pretreated with the MyD88 inhibitory peptide at 1 hpi (B) show reduced phospho-Src in comparison to infected HCF pretreated with the MyD88 control peptide. Immunoprecipitates of infected HCF at 2 hpi using antibody against Src contain MyD88 protein by immunoblot (C), and immunoprecipitates using antibody against MyD88 contain Src protein (D). Pretreatment with the MyD88 inhibitory peptide is associated with reduced signal for MyD88 in Src immunoprecipitates and reduced Src in MyD88 immunoprecipitates, with no effect seen for the MyD88 control peptide. These experiments were performed twice with similar results.