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. Author manuscript; available in PMC: 2018 Jan 31.
Published in final edited form as: Cell Rep. 2017 Jul 5;20(1):264–278. doi: 10.1016/j.celrep.2017.06.015

Figure 6. CI Assembly In Drosophila Involves An Assembly Intermediate Containing Several Membrane-Associated Accessory Subunits.

Figure 6

(A) Mitochondrial protein complexes from wildtype, mhc>dNDUFS5RNAi and mhc>dNDUFV1RNAi thoraxes were separated by BN-PAGE and the region corresponding to the accumulated assembly intermediate (demarcated) was cut out, subjected to tryptic digestion, and analyzed by label-free quantitative LC-MS/ MS.

(B) Proteomic changes in the gel slice samples from wildtype, mhc>dNDUFS5RNAi and mhc>dNDUFV1RNAi thoraxes. Relative protein abundance among biological samples is expressed by spectral counts on a log scale. The color scale bar indicates the range of protein expression levels. See additional information in Table S5.

(C) Schematic representation highlighting the membrane subunits that are upregulated in the gel slice from the mhc>dNDUFS5RNAi and mhc>dNDUFV1RNAi thoraxes.