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. Author manuscript; available in PMC: 2018 Jan 31.
Published in final edited form as: Cell Rep. 2018 Jan 16;22(3):569–575. doi: 10.1016/j.celrep.2017.12.077

Figure 3. p53 Stabilization affects System xc−.

Figure 3

(A) xCT levels following transfection (48 hr) with scrambled (SCR) siRNA or siRNA-targeting SLC7A11.

(B) Erastin2 dose-response at 48 hr, following 48 hr after transfection with siRNAs like in (A).

(C) Glutamate release, a measure of system xc− activity ± erastin2 (1 μM)following pretreatment ± nutlin-3. BD: below limit of detection.

(D) p53 and xCT protein levels following DMSO, tert-butylhydroquinone (tBHQ, 100 μM), or nutlin-3 (10 μM) treatment for 48 hr.

(E and F) SLC7A11 (E) and HMOX and GCLM (F) mRNA expression following DMSO (vehicle), nutlin-3 (10 μM), or tBHQ (100 μM) treatment for 48 hr.

Data represent mean ± SD from at least three independent biological replicates (B, C, E, and F).