Fig 4. Water block and heating assays.

(A) Water block assay was performed by floating a culture dish on a water bath in the incubator. Wire support was used to avoid movement of the dish during the assay. (B) Emission of 440 Hz, 94.0 dB, sine-wave sound resulted in the 0.4°C increase in the temperature of the culture medium in a dish (blue line). The effect of the heat itself was tested by changing temperature setting of the incubator to mimic similar heating condition (orange line). (C) Summary of the RT-qPCR result of water block (with 440 Hz, 94.0 dB, sine-wave sound) and heating (without sound) conditions. Total RNA was extracted 0 and 2 hours. (Error bars represent standard deviation, n > 5. * indicates P < 0.05 and ** indicates P < 0.01 to the 0 hour sample.).