Table 3. Calcium concentration in the bacterial pellet (Ca_b0) after pretreatment for 10 min with solutions containing 1 or 10 mM Ca, estimated binding at infinity (Ca_b∞) after equilibrium with calcium-free or 1 mM Ca solution and and half-life values calculated from kinetics release curve, for the different conditions tested (mean ± sd, n = 3).

Pretreatment	Cab0 (μmol/g pellet)	Ca release treatment	Ca b∞ (μmol/g pellet)	Half-life (min)
1 mM Ca	8.54 ± 0.26	0 mM Ca	4.24 ± 0.22	6.43
10 mM Ca	19.44 ± 0.64	0 mM Ca	7.99 ± 0.46	3.40
10 mM Ca	19.41 ± 0.58	1 mM Ca	13.06 ± 0.46	5.49