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. 2018 Jan 31;13(1):e0191805. doi: 10.1371/journal.pone.0191805

Fig 2. mTOR pathway modification in Huh7.5 cell and in primary hepatocytes.

Fig 2

A: PTP1B gene expression in Huh7.5 cells infected with JFH1 particles (1 particle/cell) and treated 24 hours post-infection with simvastatin (S: 2μM) alone or in combination with metformin (SIM-MET; S:2μM, M: 2mM). B: Gene expression of mTOR pathway in Huh7.5 cells infected with JFH1 particles and treated at 24 hours post-infection with metformin (M: 2mM) alone or in combination with simvastatin. C: Protein expression by western-blot of mTOR pathway in control or JFH1 infected in Huh 7.5 cells treated with simvastatin (SIM; 2μM), metformin (MET; 2mM) or in combination (SIM-MET; S:2μM, M: 2mM) over 72 hours. All gels have been run under the same experimental conditions. D: Protein quantification of Huh7.5 using ChemiDoc™ MP Imaging System. E: Protein expression of the mTOR pathway in human primary hepatocytes treated with simvastatin (2μM) or metformin (2mM) quantified by western-blot of three different donors. F: Protein quantification of human primary hepatocytes using ChemiDoc™ MP Imaging System. * p<0.05, ** p<0.01, ***p<0.001.