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. 2018 Jan 31;13(1):e0191805. doi: 10.1371/journal.pone.0191805

Fig 4. Autophagy modification in Huh7.5 cell and in primary hepatocytes.

Fig 4

A: MAPLC3B gene expression in Huh7.5 cells infected with JFH1 particles (1 particle/cell) and treated 24 hours post-infection with metformin (M: 2mM) alone or in combination with simvastatin (SIM-MET; S:2μM, M: 2mM). B: Western-blot analysis of autophagy markers in control or JFH1 infected in Huh 7.5 cells treated with simvastatin (SIM; 2μM), metformin (MET; 2mM) or in combination (SIM-MET; S:2μM, M: 2mM) over 72 hours. All gels have been run under the same experimental conditions. C: Protein expression of autophagy markers in human primary hepatocytes treated with simvastatin (2μM) or metformin (2mM) quantified by western-blot of three different donors. D: Protein quantification of Huh7.5 using ChemiDoc™ MP Imaging System. E: Protein quantification of human primary hepatocytes using ChemiDoc™ MP Imaging System. F: p62 protein expressions in Huh7.5 cells treated with three different concentration of metformin (M2: 2mM, M5: 5mM and M10; 10mM). * p<0.05, ** p<0.01, ***p<0.001.