Table 1. Screen hits specific to the A673 cell line (condition a) as filtered using ProFED.
| Gene symbol | Gene name | ProFED parameter | Reference a | |||
|---|---|---|---|---|---|---|
| A | B | C | ||||
| A | Hit list of more stringent ProFED profile: A ≤ -3 and B > 0 | |||||
| EPHA10 | EPH receptor 10 | -6.04 | 0.01 | (-6.06) | ||
| WNK2 | WNK lysine deficient protein kinase 2 | -3.66 | 2.11 | (-5.77) | ||
| CAMK1G | calcium/calmodulin dependent protein kinase IG | -4.42 | 1.15 | (-5.56) | ||
| BRDT | bromodomain testis associated | -3.34 | 1.70 | (-5.04) | ||
| PFKL | phosphofructokinase, liver type | -3.24 | 1.57 | (-4.81) | ||
| CDK6 | cyclin dependent kinase 6 | -3.01 | 1.77 | (-4.78) | [27] | |
| RPS6KA4 | ribosomal protein S6 kinase, polypeptide 4 | -4.24 | 0.04 | (-4.64) | ||
| CDC42BPG | CDC42 binding protein kinase gamma | -3.17 | 0.99 | (-4.16) | ||
| PDIK1L | PDLIM1 interacting kinase 1 like | -3.09 | 0.83 | (-3.92) | [28] | |
| MAP3K7 | mitogen-activated protein kinase kinase kinase 7 | -3.08 | 0.76 | (-3.84) | ||
| CDK2 | cyclin dependent kinase 2 | -3.66 | 0.09 | (-3.75) | [28,29] | |
| NRBP2 | nuclear receptor binding protein 2 | -3.11 | 0.55 | (-3.65) | ||
| PRKCD | protein kinase C delta | -3.19 | 0.26 | (-3.45) | ||
| B | Additional hits with more open ProFED profile: A ≤ -3, B open, C ≤ -3 | |||||
| DUSP21 | dual specificity phosphatase 21 | -6.20 | (-0.27) | -5.93 | [30] | |
| EPHB4 | EPH receptor B4 | -5.74 | (-0.43) | -5.31 | ||
| SRMS | SRC-related kinase lacking C-terminal regulatory kinase and N-terminal myristilation sites | -5.80 | (-0.63) | -5.17 | ||
| MAP4K2 | mitogen-activated protein kinase kinase kinase kinase 2 | -5.81 | (-0.76) | -5.05 | ||
| MAPK13 | mitogen-activated protein kinase 13 | -5.69 | (-0.71) | -4.98 | [28] | |
| PRKCE | protein kinase C epsilon | -4.73 | (-0.20) | -4.53 | ||
| PKLR | pyruvate kinase L/R | -4.60 | (-0.36) | -4.24 | [28] | |
| HK2 | hexokinase 2 | -4.06 | (-0.40) | -3.65 | ||
| BRD2 | bromodomain containing 2 | -3.92 | (-0.43) | -3.49 | ||
| CDK4 | cyclin dependent kinase 4 | -5.51 | (-2.02) | -3.48 | [28,31] | |
| PLK3 | polo like kinase 3 | -3.97 | (-0.51) | -3.46 | ||
| PKN3 | protein kinase N3 | -4.17 | (-0.78) | -3.40 | ||
| PHKG1 | phosphorylase kinase catalytic subunit gamma 1 | -3.96 | (-0.70) | -3.26 | ||
| NADK | NAD kinase | -3.79 | (-0.59) | -3.20 | ||
| TWF2 | twinfilin actin binding protein 2 | -3.07 | (-0.03) | -3.04 | ||
a previously reported as therapeutic target or respective screen hit in Ewing sarcoma.