Fig 3. Neutrophil output from BM and CNS invasion is augmented in the absence of IFNγ.

Representative flow cytometry plots depicting the gating strategy used to distinguish neutrophils (PMN) and inflammatory monocytes (IM) in the (A) BS and (B) blood of HSV-infected GKO (top row) and WT (bottom row) mice: (A) BS mononuclear cells isolated at day 4 pi show CD45^high infiltrating leukocytes, CD45^high SSC^high CD11b⁺ CD115^- Ly6G^high neutrophils (1. PMN) and F480⁺ CD115⁺ Ly6C^high monocytes (2. IMs); and (B) blood mononuclear cells at day 6 pi show SSC^high CD11b⁺ CD115^- Ly6G⁺ Ly6C^int PMN and CD115⁺ Ly6G^- Ly6C^high IMs. Bar plots depicting (C) % (left y-axis) and # (right y-axis) of CD45^high infiltrating cells in the BS of WT, IL-10KO, GKO and Rag^-/- mice at day 6 pi, (D) % (left y-axis) and # (right y-axis) of CD115⁺ F480^+/lo monocytes / macrophages within the BS CD45^high infiltrates, (E) % (left y-axis) and # (right y-axis) of Ly6C^high IMs within the BS CD45^high F480⁺ monocytes / macrophages. Line plots showing (F) % (left y-axis) and # (right y-axis) of Ly6G⁺ PMN within BS CD45^high infiltrates in GKO and WT mice at the indicated time points, (G) % CD11b⁺ Ly6G⁺ neutrophils within BM (1 leg = femur + tibia) at the indicated time points, (H) % CD11b⁺ Ly6G⁺ PMN or Ly6C^high IMs in the blood of GKO and WT mice, and (I) the ratio of PMN to IM in the blood of GKO and WT mice at the indicated time points. Data representative of 2–3 experiments are shown as mean +/- SD; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.