Fig. 2.

Body composition and whole body energy metabolism. Mice underwent body composition analysis by EchoMRI and continuous laboratory animal monitoring in individual cages at week 10. Body weight was greater in HFD+PIO mice because of greater fat mass (A). ANCOVA revealed that PIO treatment did not change the positive relationship between total daily energy expenditure (EE) and lean body mass during 24 h of feeding (B). PIO treatment resulted in greater energy expenditure during 24 h of fasting without an interaction with lean body mass (C). Whole body respiratory exchange ratio (RER) was lower with HFD with no effect of PIO (D). Body composition and RER data were compared with two-way ANOVA (diet × drug) with post hoc analysis performed using Tukey’s test. Bars indicate means  ± SD; n = 8–14 per group. ***P < 0.01 for post hoc comparison within diet group; #P < 0.0001 between intercepts of regression lines.