Fig. 4.

Energy expenditure (EE; A) and respiratory quotient (RQ; B) scores over 72 h postinjection for separate groups of rats (n = 6) microinjected into the 3V with single equimolar doses of DET (0, 0.5, and 1.0 mU) or regular insulin (0, 2.0 and 4.0 mU) in ascending order in a volume of 1.0 µl. aCSF served as the vehicle for the insulin and was used as the 0-mU dose. Doses were administered at 1-wk intervals. Following administration of each dose, rates of O₂ utilization and CO₂ production were measured for a 3-h period each 24 h over 24-72 h and used for calculation of EE and RQ values of the animals. Baseline (0 h) measurements were obtained in a 3-h session before microinjection of each dose. Food was not available in the metabolic chambers during testing. Group mean EE and RQ values are represented. A: *P < 0.05, different from aCSF baseline; **P < 0.01, different from aCSF baseline; #P < 0.05, different from 4.0 mU regular insulin; ##P < 0.01, different from 4.0 mU regular insulin; §P < 0.05, different from 2.0 mU regular insulin. B: *P < 0.05, different from aCSF baseline; **P < 0.01, different from aCSF baseline; #P < 0.05, different from 4.0 mU regular insulin; ##P < 0.01, different from 4.0 mU regular insulin; §P < 0.05, different from 2.0 mU regular insulin.