Fig. 5.

Feeding by groups of rats in response to two sequential microinjections of vehicle, 0.25 or 0.5 mU of DET and vehicle, ghrelin (100 pmol) and vehicle, or combinations of DET and ghrelin. Microinjections were administered to two separate groups at 3V (A) or paraventricular nucleus (PVN) (B) sites (n = 7 and 8, respectively). Two microinjections separated by 60 min were made, with feeding monitored for 4 h following the 2nd microinjection. Injection combinations were as follows: V/V, vehicle/vehicle; D.25/V, 0.25 mU DET/vehicle; D.5/V, 0.5 mU DET/vehicle; V/g, vehicle/ghrelin; D.25/g, 0.25 mU DET/ghrelin; D.5/g, 0.5 mU DET/ghrelin. Significance levels are as follows: *P < 0.05, different from V/V; **P < 0.01, different from V/V; ##P < 0.01, different from V/g.