Fig. 7.

Effects of arginase inhibitor or A1 knockdown on A1 expression/activity and nitric oxide (NO) production in HG + PA-incubated endothelial cells. A1 mRNA (A) and protein expression (B and C) and arginase activity (D) with arginase inhibitor treatment and A1 knockdown by CRISPR/Cas 9-transduction of sgRNA with a 48-h exposure to normal control media (CTRL) or media with high glucose (HG; 25 mM) and palmitate (PA; 200 μM). NO production with and without ABH and nitric oxide synthase inhibitor [nitro-l-arginine methyl ester (l-NAME)] treatment and A1 downregulation with 48-h exposure to CTRL or HG + PA media (E). Values are means ± SE; n = 4–6 experiments. *P < 0.05 vs. vehicle-treated (Veh) cells or vector-transfected cells in control media.