Table 3.

Fold changes in mRNA of chemokines and adhesion molecules in mouse aortic endothelial cells

	Veh		ABH			Vector		A1-sgRNA
	CTRL	HG + PA	CTRL	HG + PA	ABH + l-NAME	CTRL	HG + PA	CTRL	HG + PA	A1-sgRNA + l-NAME
MCP-1	1.03 ± 0.05	4.96 ± 0.54*	0.97 ± 0.03	1.87 ± 0.23*#	3.03 ± 0.39*	1.02 ± 0.08	5.04 ± 0.56*	0.63 ± 0.21	1.89 ± 0.26*#	3.13 ± 0.48*
ICAM-1	1.03 ± 0.08	5.00 ± 0.58*	1.05 ± 0.13	1.26 ± 0.21	3.48 ± 0.46*	1.04 ± 0.20	4.18 ± 0.66*	1.00 ± 0.11	1.40 ± 0.19	3.01 ± 0.51*
VCAM-1	1.07 ± 0.11	11.74 ± 2.71*	1.18 ± 0.13	1.90 ± 0.47	10.95 ± 2.03*	1.05 ± 0.15	12.14 ± 2.57*	1.13 ± 0.14	1.94 ± 0.42	9.66 ± 2.02*

Values are means ± SE; n = 5 experiments. mRNA levels of MCP-1, ICAM-1, and V-CAM-1 in mouse aortic endothelial cells (MAECs) with and without arginase inhibitor (ABH), nitric oxide synthase inhibitor [nitro-l-arginine methyl ester (l-NAME)] treatment, or A1 downregulation by CRISPR/Cas 9-transduction of sgRNA after a 48-h incubation in normal media (CTRL) or media containing high glucose and palmitate (HG + PA).

^*P < 0.05 vs. CTRL vehicle or vector-transfected groups.

^#P < 0.05 HG + PA + ABH or A1-sgRNA HG + PA vs. Veh HG + PA or vector-transfected HG + PA groups, respectively.