Table 4.
mRNA fold changes of cytokines in mouse aorta endothelial cells
| Veh |
ABH |
Vector |
A1-sgRNA |
|||||
|---|---|---|---|---|---|---|---|---|
| CTRL | HG + PA | CTRL | HG + PA | CTRL | HG + PA | CTRL | HG + PA | |
| TNF-α | 1.02 ± 0.10 | 13.9 ± 1.89* | 1.17 ± 0.36 | 14.9 ± 1.31* | 1.03 ± 0.10 | 13.3 ± 2.46* | 0.95 ± 0.11 | 11.80 ± 2.28* |
| IL-10 | 1.01 ± 0.12 | 1.66 ± 0.05* | 0.99 ± 0.15 | 1.58 ± 0.15* | 1.02 ± 0.17 | 1.72 ± 0.09* | 1.08 ± 0.21 | 1.77 ± 0.13* |
Values are means ± SE; n = 5 experiments. mRNA levels of TNF-α and IL-10 in MAECs with and without arginase inhibitor (ABH) or ARG1 knockdown by CRISPR/Cas 9 transduction of sgRNA after 48 h incubation in normal media (CTRL) or media containing high glucose and palmitate (HG + PA).
*
P < 0.05 vs. CTRL vehicle or vector-transfected groups.