Fig. 3.

TRPV4 channels contribute to stretch-induced intracellular Ca²⁺ concentration ([Ca²⁺]_i) elevation and constriction in neonatal pig renal vascular SMCs. Traces (A) and bar graphs (B) showing changes in [Ca²⁺]_i induced by transmembrane hyposmotic challenge (n = 5) in control and SMCs pretreated with GsMTx4 (300 nM; n = 4), nimodipine (1 µM; n = 4), HC 067047 (1 µM; n = 5), or OBAA (1 µM; n = 4). C–F, traces and bar graphs demonstrating that hyposmotic challenge-induced constriction of neonatal pig renal interlobular arteries is inhibited by HC 067047 (1 µM; n = 7 each) but not OBAA (1 µM; n = 5 each). Cells/vessels were pretreated with inhibitors/blockers 10–15 min before hyposmotic challenge. *P < 0.05 vs. control (hyposmotic).