Table 1.

Oligonucleotide primer sequences

Gene	Sequence	Accession	Length, bp
RT-PCR
ACTA2		NM_001164650.1	456
Forward	5′-CCCTGTGAAGCACCAGCCAGGA-3′
Reverse	5′-GTAGAGGGACAGCACCGCCTGA-3′
vWR		NM_001246221.1	488
Forward	5′-CGTGGAGAGTGCTGAGTGTT-3′
Reverse	5′-GGCCATCCCAGTCTATCTGC-3′
TRPV1		EF100781.1*	167
Forward	5′-TGTTCAAGTTCACCATCGGC-3′
Reverse	5′-CTCCTGTGCGATCTTGTTGAC-3′
TRPV2		XM_013980349.1#	444
Forward	5′- CAGGCTTGAGACGTCAGACA-3′
Reverse	5′-TCTGTACACTGGGCGTTGAC-3′
TRPV3		XM_005669116.2#	211
Forward	5′-CCAACATCGACAACCGGCAT-3′
Reverse	5′-CACCCCATTTTGTGCGTGAG-3′
TRPV4		NM_001130729.1	298
Forward	5′-CTTCGCTGGCCAACCTGTTT-3′
Reverse	5′-TGCGGCTGCTTCTCTATGAC-3′
Quantitative RT-PCR
18S rRNA		NR_046261.1	102
Forward	5′-CGAAAGCATTTGCCAAGAAT-3′
Reverse	5′-AGTCGGCATCGTTTATGGTC-3′
TRPV4		NM_001130729.1	105
Forward	5′-CCGCTTACTACCAGCCTCTG-3′
Reverse	5′-GGACCCCAGTGAAGAGTGTG-3′

vWR, von Willebrand factor; TRPV, transient receptor potential vanilloid.

^*Partial sequence.

^#Predicted sequences.