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. 2017 Aug 9;313(5):C501–C515. doi: 10.1152/ajpcell.00075.2017

Fig. 5.

Fig. 5.

Localization of eIF4E-BP1-P-S64, eIF4E-BP1-P-S111, and PLK1 signal mouse oocyte MII spindle after DMSO, BI2536, Torin 1, and KU55933 treatment observed by IFCM. A–L: MII oocytes were treated with DMSO, BI2536, Torin 1, or KU55933 for 3 h in vitro, fixed, and immunostained. A–D: eIF4E-BP1-P-S111 spindle localization in MII oocytes matured in DMSO, BI2536, Torin 1, or KU55933. E–H: eIF4E-BP1-P-S64 spindle localization in MII oocytes matured in DMSO, BI2536, Torin 1, or KU55933. I–L: PLK1 spindle localization in MII oocytes matured in DMSO, BI2536, Torin 1, or KU55933. M and N: MII oocytes treated with DMSO (M) or BI2536 (N) were stained for total eIF4E-BP1. This pan-eIF4E-BP1 staining was examined in each confocal section encompassing the spindle to determine whether void or difference in signal. Two representative Z-stacks are shown for each group. Quantification for no. of oocytes with target protein localized to spindle out of total oocytes quantified shown below each images. Oocytes were collected from 5 BDF1 mice, pooled, and randomly assigned to either DMSO or inhibitor treatment groups; this was repeated for a minimum of 2 replicates. Bar, 10 μm.