Fig. 5.

Osteoclast fusion gene expression following muscle paralysis. Whole bone marrow was flushed from the right tibia of naïve mice and mice that had received an IM injection of BTxA in the right calf 1, 3, and 7 days previously. After preparation of total RNA and synthesis of cDNA, quantitative RT-PCR was performed using DC-STAMP and OC-STAMP gene-specific primers. Gene expression levels were quantified relative to the housekeeping gene HPRT. Values represent means ± SE (n = 6 per group), *P < 0.05 vs. naïve.