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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: J Cell Physiol. 2017 Oct 4;233(4):3230–3243. doi: 10.1002/jcp.26166

Figure 4.

Figure 4

Opposing effects of extracellular nucleotides on TNAP activity in VSMCs and osteoblasts. (a) ATP, (b) UTP, and (c) PPi (≥1 μM) increased VSMC TNAP activity by up to 88%. (d) ATP and (e) UTP reduced osteoblast TNAP activity by up to 50%. (f) No effect of PPi on osteoblast TNAP activity. Note that the basal levels of TNAP activity are approximately 100-fold higher in mineralizing osteoblasts than calcifying VSMCs. Values are mean ± SEM (n = 6), *p < 0.05, **p <0.01, *** p< 0.001